Phenylacetaldoxime dehydratase explained

Phenylacetaldoxime dehydratase
Ec Number:	4.99.1.7

In enzymology, a phenylacetaldoxime dehydratase is an enzyme that catalyzes the chemical reaction

(Z)-phenylacetaldehyde oxime

\rightleftharpoons phenylacetonitrile + H₂O

Hence, this enzyme has one substrate, (Z)-phenylacetaldehyde oxime, and two products, phenylacetonitrile and H₂O.

This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that do not fit into any other sub-class. The systematic name of this enzyme class is (Z)-phenylacetaldehyde-oxime hydro-lyase (phenylacetonitrile-forming). Other names in common use include PAOx dehydratase, arylacetaldoxime dehydratase, OxdB, and (Z)-phenylacetaldehyde-oxime hydro-lyase. This enzyme participates in styrene degradation.

References

• Kato Y, Nakamura K, Sakiyama H, Mayhew SG, Asano Y . 2000 . Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene . Biochemistry . 39 . 800 - 9 . 10651646 . 10.1021/bi991598u . 4 .
• Kobayashi K, Yoshioka S, Kato Y, Asano Y, Aono S . 2005 . Regulation of aldoxime dehydratase activity by redox-dependent change in the coordination structure of the aldoxime-heme complex . J. Biol. Chem. . 280 . 5486 - 90 . 15596434 . 10.1074/jbc.M410474200 . 7 . free .