Peptidylglycine monooxygenase explained

peptidylglycine monooxygenase
Ec Number:	1.14.17.3
Cas Number:	90597-47-0
Go Code:	0004504

In enzymology, a peptidylglycine monooxygenase is an enzyme that catalyzes the chemical reaction

peptidylglycine + ascorbate + O₂

\rightleftharpoons peptidyl(2-hydroxyglycine) + dehydroascorbate + H₂O

The 3 substrates of this enzyme are peptidylglycine, ascorbate, and O₂, whereas its 3 products are peptidyl(2-hydroxyglycine), dehydroascorbate, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is . Other names in common use include , , , , , , , , PAM-A, PAM-B, and PAM. It employs one cofactor, copper.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,, and .

References

• Bradbury AF, Finnie MD, Smyth DG . 1982 . Mechanism of C-terminal amide formation by pituitary enzymes . Nature . 298 . 686 - 8 . 7099265 . 10.1038/298686a0 . 5875 . 1982Natur.298..686B . 4324776 .
• Bradbury AF, Smyth DG . 1987 . Enzyme-catalysed peptide amidation. Isolation of a stable intermediate formed by reaction of the amidating enzyme with an imino acid . Eur. J. Biochem. . 169 . 579 - 84 . 3691506 . 10.1111/j.1432-1033.1987.tb13648.x . 3 . free .
• Glembotski CC . 1985 . Further characterization of the peptidyl alpha-amidating enzyme in rat anterior pituitary secretory granules . Arch. Biochem. Biophys. . 241 . 673 - 83 . 2994573 . 10.1016/0003-9861(85)90594-6 . 2 .
• Katopodis AG, Ping D, May SW . 1990 . A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of alpha-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine alpha-amidating monooxygenase in peptide amidation . Biochemistry . 29 . 6115 - 20 . 2207061 . 10.1021/bi00478a001 . 26 .
• Murthy AS, Keutmann HT, Eipper BA . 1987 . Further characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary . Mol. Endocrinol. . 1 . 290 - 9 . 3453894 . 10.1210/mend-1-4-290 . 4 . free .
• Murthy AS, Mains RE, Eipper BA . 1986 . Purification and characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary . J. Biol. Chem. . 261 . 1815 - 22 . 3944110 . 4 .