Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase explained
| peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase |
| Ec Number: | 3.5.1.52 |
| Cas Number: | 83534-39-8 |
| Go Code: | 0000224 |
In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.
The NGLY1 gene encodes the ortholog of this enzyme in humans.
Nomenclature
The systematic name of this enzyme class is N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase. Other names in common use include:
- glycopeptide N-glycosidase,
- glycopeptidase,
- N-oligosaccharide glycopeptidase,
- N-glycanase,
- Jack-bean glycopeptidase,
- PNGase A,[1] and
- PNGase F
Structural studies
The enzyme uses a catalytic triad of cysteine-histidine-aspartate in its active site for hydrolysis by covalent catalysis.[2] A peptide with similar functionality was discovered in 2014 by group at Fudan University in Shanghai, China. This peptide also cleaves alpha 1,3 linkages, and has been named PNGase F-II.[3]
Further reading
- Plummer TH, Tarentino AL . Facile cleavage of complex oligosaccharides from glycopeptides by almond emulsin peptide: N-glycosidase . The Journal of Biological Chemistry . 256 . 20 . 10243–6 . Oct 1981 . 10.1016/S0021-9258(19)68610-2 . 7287707 . free .
- Takahashi N . Demonstration of a new amidase acting on glycopeptides . Biochemical and Biophysical Research Communications . 76 . 4 . 1194–201 . Jun 1977 . 901470 . 10.1016/0006-291X(77)90982-2 .
- Takahashi N, Nishibe H . Some characteristics of a new glycopeptidase acting on aspartylglycosylamine linkages . Journal of Biochemistry . 84 . 6 . 1467–73 . Dec 1978 . 738997 . 10.1093/oxfordjournals.jbchem.a132270.
- Tarentino AL, Gómez CM, Plummer TH . Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F . Biochemistry . 24 . 17 . 4665–71 . Aug 1985 . 4063349 . 10.1021/bi00338a028 .
Notes and References
- Altmann F, Paschinger K, Dalik T, Vorauer K . Characterisation of peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A and its N-glycans . European Journal of Biochemistry . 252 . 1 . 118–23 . Feb 1998 . 9523720 . 10.1046/j.1432-1327.1998.2520118.x . free .
- Allen MD, Buchberger A, Bycroft M . The PUB domain functions as a p97 binding module in human peptide N-glycanase . The Journal of Biological Chemistry . 281 . 35 . 25502–8 . Sep 2006 . 16807242 . 10.1074/jbc.M601173200 . free .
- Sun G, Yu X, Bao C, Wang L, Li M, Gan J, Qu D, Ma J, Chen L . Identification and characterization of a novel prokaryotic peptide: N-glycosidase from Elizabethkingia meningoseptica . The Journal of Biological Chemistry . 290 . 12 . 7452–62 . Mar 2015 . 25614628 . 10.1074/jbc.M114.605493 . 4367255. free .
