Pentachlorophenol monooxygenase explained

Pentachlorophenol monooxygenase
Ec Number:	1.14.13.50
Cas Number:	136111-57-4

Pentachlorophenol monooxygenase (pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol 4-monooxygenase, PCP hydroxylase, pentachlorophenol hydroxylase, PcpB, PCB 4-monooxygenase, PCB4MO) is an enzyme with systematic name pentachlorophenol,NADPH:oxygen oxidoreductase (hydroxylating, dechlorinating).^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

(1) pentachlorophenol + 2 NADPH + H⁺ + O2

\rightleftharpoons 2,3,5,6-tetrachlorohydroquinone + 2 NADP⁺ + chloride + H₂O

(2) 2,3,5,6-tetrachlorophenol + NADPH + H⁺ + O2

\rightleftharpoons 2,3,5,6-tetrachlorohydroquinone + NADP⁺ + H₂O

Pentachlorophenol monooxygenase is a flavoprotein (FAD).

Notes and References

1. Schenk T, Müller R, Mörsberger F, Otto MK, Lingens F . Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790 . Journal of Bacteriology . 171 . 10 . 5487–91 . October 1989 . 10.1128/jb.171.10.5487-5491.1989 . 2793827 . 210388 .
2. Xun L, Topp E, Orser CS . Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries . Journal of Bacteriology . 174 . 9 . 2898–902 . May 1992 . 10.1128/jb.174.9.2898-2902.1992 . 1569020 . 205942 .
3. Xun L, Topp E, Orser CS . Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase . Journal of Bacteriology . 174 . 17 . 5745–7 . September 1992 . 10.1128/jb.174.17.5745-5747.1992 . 1512208 . 206524 .
4. Lange CC, Schneider BJ, Orser CS . Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723 . Biochemical and Biophysical Research Communications . 219 . 1 . 146–9 . February 1996 . 8619798 . 10.1006/bbrc.1996.0196 .
5. Nakamura T, Motoyama T, Hirono S, Yamaguchi I . Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics . 1700 . 2 . 151–9 . August 2004 . 15262224 . 10.1016/j.bbapap.2004.04.008 .