Orotate reductase (NADH) explained

orotate reductase (NADH)
Ec Number:	1.3.1.14
Cas Number:	37255-26-8
Go Code:	0004589

In enzymology, an orotate reductase (NADH) is an enzyme that catalyzes the chemical reaction

(S)-dihydroorotate + NAD⁺

\rightleftharpoons orotate + NADH + H⁺

Thus, the two substrates of this enzyme are (S)-dihydroorotate and NAD⁺, whereas its 3 products are orotate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (S)-dihydroorotate:NAD+ oxidoreductase. This enzyme is also called orotate reductase (NADH). This enzyme participates in pyrimidine metabolism. It has 2 cofactors: FAD, and FMN.

References

• FRIEDMANN HC, VENNESLAND B . 1958 . Purification and properties of dihydro-orotic dehydrogenase . J. Biol. Chem. . 233 . 1398 - 406 . 13610849 . 6 .
• FRIEDMANN HC, VENNESLAND B . 1960 . Crystalline dihydroorotic dehydrogenase . J. Biol. Chem. . 235 . 1526 - 32 . 13825167 .
• LIEBERMAN I, KORNBERG A . 1953 . Enzymic synthesis and breakdown of a pyrimidine, orotic acid. I Dihydro-orotic dehydrogenase . Biochim. Biophys. Acta . 12 . 223 - 34 . 13115431 . 10.1016/0006-3002(53)90141-3 . 1–2 .