Opine dehydrogenase explained

opine dehydrogenase
Ec Number:	1.5.1.28
Cas Number:	108281-02-3
Go Code:	0047129

In enzymology, an opine dehydrogenase is an enzyme that catalyzes the chemical reaction

(2S)-2-pentanoate + NAD⁺ + H₂O

\rightleftharpoons L-2-aminopentanoic acid + pyruvate + NADH + H⁺

The 3 substrates of this enzyme are (2S)-2-pentanoate, NAD⁺, and H₂O, whereas its 4 products are L-2-aminopentanoic acid, pyruvate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (2S)-2-pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming). Other names in common use include (2S)-2-pentanoate dehydrogenase (NAD+,, and L-aminopentanoate-forming).

References

• Asano Y, Yamaguchi K, Kondo K . 1989 . A new NAD+-dependent opine dehydrogenase from Arthrobacter sp strain 1C . J. Bacteriol. . 171 . 4466 - 71 . 2753861 . 8 . 210226 .
• Dairi T, Asano Y . 1995 . Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C . Appl. Environ. Microbiol. . 61 . 3169 - 71 . 7487048 . 8 . 167592 .
• Kato Y, Yamada H, Asano Y . 1996 . Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes . J. Mol. Catal., B Enzym. . 1 . 151 - 160 . 10.1016/1381-1177(95)00011-9 . 3–6 .