Nuclear-inclusion-a endopeptidase explained

Nuclear-inclusion-a endopeptidase
Ec Number:	3.4.22.44
Cas Number:	139946-51-3

Nuclear-inclusion-a endopeptidase (potyvirus NIa protease) is a protease enzyme found in potyviruses.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction:

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.

e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).

Notes and References

1. The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection . Fellers, J.P. . Collins, G.B. . Hunt, A.G. . Crop Sci. . 1998 . 38 . 1309–1319 . 10.2135/cropsci1998.0011183x003800050030x.
2. Book: Potyvirus NIa protease . Handbook of Proteolytic Enzymes . Kim, D.-H. . Choi, K.Y. . 1998 . 721–723 . Barrett, A.J. . Rawlings, N.D. . Woessner, J.F. . Academic Press . London .
3. Takahashi T, Nakanishi M, Yao Y, Uyeda I, Serizawa N . Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli . Bioscience, Biotechnology, and Biochemistry . 62 . 5 . 953–8 . May 1998 . 9648226 . 10.1271/bbb.62.953 .
4. Kim DH, Hwang DC, Kang BH, Lew J, Han J, Song BD, Choi KY . Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity . Virology . 226 . 2 . 183–90 . December 1996 . 8955037 . 10.1006/viro.1996.0645 . free .