Nitronate monooxygenase explained

Nitronate monooxygenase
Ec Number:	1.13.12.16

Nitronate monooxygenase (NMO) is an enzyme with systematic name nitronate:oxygen 2-oxidoreductase (nitrite-forming).^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

ethylnitronate + O₂

\rightleftharpoons acetaldehyde + nitrite + other products

The enzymes from the fungus Neurospora crassa and the yeast Williopsis saturnus var. mrakii contain non-covalently bound FMN as the cofactor.

Notes and References

1. Francis K, Russell B, Gadda G . Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase . The Journal of Biological Chemistry . 280 . 7 . 5195–204 . February 2005 . 15582992 . 10.1074/jbc.M411249200 . free .
2. Ha JY, Min JY, Lee SK, Kim HS, Kim DJ, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW . Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base . The Journal of Biological Chemistry . 281 . 27 . 18660–7 . July 2006 . 16682407 . 10.1074/jbc.M601658200 . free .
3. Gadda G, Francis K . Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis . Archives of Biochemistry and Biophysics . 493 . 1 . 53–61 . January 2010 . 19577534 . 10.1016/j.abb.2009.06.018 .
4. Francis K, Gadda G . Kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase . Bioorganic Chemistry . 37 . 5 . 167–72 . October 2009 . 19683782 . 10.1016/j.bioorg.2009.07.005 .