Nitroalkane oxidase explained

nitroethane oxidase
Ec Number:	1.7.3.1
Cas Number:	9029-36-1
Go Code:	0050141

In enzymology, a nitroalkane oxidase is an enzyme that catalyzes the chemical reaction

a nitroalkane + H₂O + O₂

\rightleftharpoons an aldehyde or ketone + nitrite + H₂O₂

The 3 substrates of this enzyme are nitroalkane, H₂O, and O₂, whereas its 4 products are aldehyde, ketone, nitrite, and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with oxygen as acceptor. The systematic name of this enzyme class is nitroalkane:oxygen oxidoreductase. Other names in common use include nitroethane oxidase, NAO, and nitroethane:oxygen oxidoreductase. This enzyme participates in nitrogen metabolism.

References

• LITTLE HN . 1951 . Oxidation of nitroethane by extracts from Neurospora . J. Biol. Chem. . 193 . 347 - 58 . 14907722 . 1 . 10.1016/S0021-9258(19)52460-7 . free .
• Kido T, Hashizume K, Soda K . 1978 . Purification and properties of nitroalkane oxidase from Fusarium oxysporum . J. Bacteriol. . 133 . 53 - 8 . 22538 . 1 . 10.1128/jb.133.1.53-58.1978 . 221975 .
• Daubner SC, Gadda G, Valley MP, Fitzpatrick PF . 2002 . Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily . Proc. Natl. Acad. Sci. U.S.A. . 99 . 2702 - 7 . 11867731 . 10.1073/pnas.052527799 . 5 . 122411 . free .
• Fitzpatrick PF, Orville AM, Nagpal A, Valley MP . 2005 . Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase . Arch. Biochem. Biophys. . 433 . 157 - 65 . 15581574 . 10.1016/j.abb.2004.08.021 . 1 .
• Valley MP, Tichy SE, Fitzpatrick PF . 2005 . Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase . J. Am. Chem. Soc. . 127 . 2062 - 6 . 15713081 . 10.1021/ja043542f . 7 . 1630679 .