Nitrite reductase (NO-forming) explained
| nitrite reductase (NO-forming) |
| Ec Number: | 1.7.2.1 |
| Cas Number: | 37256-41-0 |
| Go Code: | 0050421 |
| Width: | 270 |
In enzymology, a nitrite reductase (NO-forming) is an enzyme that catalyzes the chemical reaction
nitric oxide + H2O + ferricytochrome c ⇌ nitrite + ferrocytochrome c + 2 H+
The 3 substrates of this enzyme are nitric oxide, H2O, and ferricytochrome c, whereas its 3 products are nitrite, ferrocytochrome c, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with a cytochrome as acceptor. The systematic name of this enzyme class is nitric-oxide:ferricytochrome-c oxidoreductase. Other names in common use include cd-cytochrome nitrite reductase, [nitrite reductase (cytochrome)] [misleading, see comments.], cytochrome c-551:O2, NO2+ oxidoreductase, cytochrome cd, cytochrome cd1, hydroxylamine (acceptor) reductase, methyl viologen-nitrite reductase, nitrite reductase (cytochrome, and NO-forming). This enzyme participates in nitrogen metabolism. It has 3 cofactors: FAD, Iron, and Copper.
Structural studies
As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,,,,,,,,,,,,, and .
References
- Miyata M, Mori T . Tokyo . Studies on denitrification. X. The "denitrifying enzyme" as a nitrite reductase and the electron donating system for denitrification . J. Biochem. . 66. 463–471 . 5354021 . 4 . October 1969. 10.1093/oxfordjournals.jbchem.a129170 .
- CHUNG CW, NAJJAR VA . 1956 . Cofactor requirements for enzymatic denitrification. I. Nitrite reductase . J. Biol. Chem. . 218 . 617–625 . 13295215 . 2 . 10.1016/S0021-9258(18)65827-2 . free .
- Walker GC, Nicholas DJ . 1961 . Nitrite reductase from Pseudomonas aeruginosa . Biochim. Biophys. Acta . 13782716 . 49 . 2 . 350–360 . 10.1016/0006-3002(61)90134-2 .
- Singh J . 1974 . Cytochrome oxidase from Pseudomonas aeruginosa. III. Reduction of hydroxylamine . Biochim. Biophys. Acta . 333 . 1 . 28–36 . 19396990 . 10.1016/0005-2728(74)90159-5.
- 10.1016/0167-4838(85)90048-2 . Michalski WP, Nicholas DJ . 1985 . Molecular characterization of a copper-containing nitrite reductase from Rhodopseudomonas sphaeriodes forma sp. Denitrificans . Biochim. Biophys. Acta . 828 . 2 . 130–137 .
- Godden JW . 1991 . The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes . Science . 253 . 438–442 . 1862344 . 10.1126/science.1862344 . Turley . S . Teller . DC . Adman . ET . Liu . MY . Payne . WJ . Legall . J . 5018 . 1991Sci...253..438G .
- Williams PA, Fülöp V, Leung YC, Chan C, Moir JW, Howlett G, Ferguson SJ, Radford SE, Hajdu J. 1995 . Pseudospecific docking surfaces on electron transfer proteins as illustrated by pseudoazurin, cytochrome c550 and cytochrome cd1 nitrite reductase . Nat. Struct. Biol. . 2 . 975–982 . 7583671 . 10.1038/nsb1195-975 . 11 . 21798842 .
- B, Kroneck PM . 1996 . Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans . Arch. Microbiol. . 165 . 55–61 . 8639023 . 10.1007/s002030050296 . Vollack . KU . Zumft . WG . Eisenmann . E . Siddiqui . RA . Friedrich . B . Kroneck . PM . 1 . 12737971 .
- Zumft WG . 1997 . Cell biology and molecular basis of denitrification . Microbiol. Mol. Biol. Rev. . 61 . 533–616 . 9409151 . 4 . 10.1128/mmbr.61.4.533-616.1997 . 232623 .
- Ferguson SJ . 1998 . Nitrogen cycle enzymology . Curr. Opin. Chem. Biol. . 2 . 182–193 . 9667932 . 10.1016/S1367-5931(98)80059-8 . 2 .
- 10.1099/00221287-143-9-2853 . Vijgenboom E, Busch JE, Canters GW . September 1997. In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression is under control of rpoS and ANR . Microbiology . 143 . 9 . 2853–2863 . 9308169 . free . 1887/3239420 . free .
