Nitrite reductase (NAD(P)H) explained

nitrite reductase [NAD(P)H]
Ec Number:	1.7.1.4
Cas Number:	9029-29-2
Go Code:	0008942

In enzymology, a nitrite reductase [NAD(P)H] is an enzyme that catalyzes the chemical reaction

ammonium hydroxide + 3 NAD(P)⁺ + H₂O

\rightleftharpoons nitrite + 3 NAD(P)H + 3 H⁺

The 4 substrates of this enzyme are ammonium hydroxide, NAD⁺, NADP⁺, and H₂O, whereas its 4 products are nitrite, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is ammonium-hydroxide:NAD(P)+ oxidoreductase. Other names in common use include nitrite reductase (reduced nicotinamide adenine dinucleotide, (phosphate)), NADH-nitrite oxidoreductase, NADPH-nitrite reductase, assimilatory nitrite reductase, nitrite reductase [NAD(P)H2], and NAD(P)H2:nitrite oxidoreductase. This enzyme participates in nitrogen metabolism. It has 3 cofactors: FAD, Iron, and Siroheme.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .

References

• Athel Cornish-Bowden. Cammack R, Jackson RH, Cornish-Bowden A, Cole JA . 1982 . Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12 . Biochem. J. . 207 . 333 - 9 . 6297458 . 2 . 10.1042/bj2070333 . 1153865 .
• Lazzarini RA . Atkinson DE . 1961 . A triphosphopyridine nucleotide-specific nitrite reductase from Escherichia coli . J. Biol. Chem. . 236 . 12 . 3330 - 3335 . 10.1016/S0021-9258(18)94019-6 . 14463130. free .
• Nicholas DJ, Medina A, Jones OT . 1960 . A nitrite reductase from Neurospora crassa . Biochim. Biophys. Acta . 37 . 3 . 468–76 . 10.1016/0006-3002(60)90503-5 . 14426899 .
• Taniguchi H, Mitsui H, Nakamura K, Egami F . Ann. Acad. Sci. Fenn. Ser. A II 60 (1955) 200 .
• Colandene JD, Garrett RH . 1996 . Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa . J. Biol. Chem. . 271 . 24096 - 104 . 8798648 . 10.1074/jbc.271.39.24096 . 39 . free .