Nitric oxide reductase (cytochrome c) explained

Nitric oxide reductase (cytochrome c)
Ec Number:	1.7.2.5

Nitric oxide reductase (cytochrome c) is an enzyme with systematic name nitrous oxide:ferricytochrome-c oxidoreductase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

2 nitric oxide + 2 ferrocytochrome c + 2 H⁺

\rightleftharpoons nitrous oxide + 2 ferricytochrome c + H₂O

The enzyme from Pseudomonas aeruginosa contains a dinuclear centre.

Notes and References

1. Hendriks J, Warne A, Gohlke U, Haltia T, Ludovici C, Lübben M, Saraste M . The active site of the bacterial nitric oxide reductase is a dinuclear iron center . Biochemistry . 37 . 38 . 13102–9 . September 1998 . 9748316 . 10.1021/bi980943x .
2. Hendriks J, Gohlke U, Saraste M . From NO to OO: nitric oxide and dioxygen in bacterial respiration . Journal of Bioenergetics and Biomembranes . 30 . 1 . 15–24 . February 1998 . 9623801 . 10.1023/A:1020547225398 .
3. Heiss B, Frunzke K, Zumft WG . Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri . Journal of Bacteriology . 171 . 6 . 3288–97 . June 1989 . 2542222 . 210048 .
4. Cheesman MR, Zumft WG, Thomson AJ . The MCD and EPR of the heme centers of nitric oxide reductase from Pseudomonas stutzeri: evidence that the enzyme is structurally related to the heme-copper oxidases . Biochemistry . 37 . 11 . 3994–4000 . March 1998 . 9521721 . 10.1021/bi972437y .
5. Kumita H, Matsuura K, Hino T, Takahashi S, Hori H, Fukumori Y, Morishima I, Shiro Y . NO reduction by nitric-oxide reductase from denitrifying bacterium Pseudomonas aeruginosa: characterization of reaction intermediates that appear in the single turnover cycle . The Journal of Biological Chemistry . 279 . 53 . 55247–54 . December 2004 . 15504726 . 10.1074/jbc.M409996200 . free .
6. Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y . Structural basis of biological N2O generation by bacterial nitric oxide reductase . Science . 330 . 6011 . 1666–70 . December 2010 . 21109633 . 10.1126/science.1195591 .