Nitrate reductase (quinone) explained

Nitrate reductase (quinone)
Ec Number:	1.7.5.1

Nitrate reductase (quinone) (nitrate reductase A, nitrate reductase Z, quinol/nitrate oxidoreductase, quinol-nitrate oxidoreductase, quinol:nitrate oxidoreductase, NarA, NarZ, NarGHI) is an enzyme with systematic name nitrite:quinone oxidoreductase.^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

\rightleftharpoons nitrite + a quinone + H₂O

This is a membrane-bound enzyme which supports [anaerobic respiration] on nitrate.

Notes and References

1. Enoch HG, Lester RL . The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli . Biochemical and Biophysical Research Communications . 61 . 4 . 1234–41 . December 1974 . 4616697 . 10.1016/s0006-291x(74)80416-x .
2. Bertero MG, Rothery RA, Palak M, Hou C, Lim D, Blasco F, Weiner JH, Strynadka NC . Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A . Nature Structural Biology . 10 . 9 . 681–7 . September 2003 . 12910261 . 10.1038/nsb969 .
3. Lanciano P, Magalon A, Bertrand P, Guigliarelli B, Grimaldi S . High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD . Biochemistry . 46 . 18 . 5323–9 . May 2007 . 17439244 . 10.1021/bi700074y .
4. Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC . Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A . The Journal of Biological Chemistry . 280 . 15 . 14836–43 . April 2005 . 15615728 . 10.1074/jbc.M410457200 . free .
5. Bonnefoy V, Demoss JA . Nitrate reductases in Escherichia coli . Antonie van Leeuwenhoek . 66 . 1-3 . 47–56 . 1994 . 7747940 . 10.1007/bf00871632 .
6. Guigliarelli B, Asso M, More C, Augier V, Blasco F, Pommier J, Giordano G, Bertrand P . EPR and redox characterization of iron-sulfur centers in nitrate reductases A and Z from Escherichia coli. Evidence for a high-potential and a low-potential class and their relevance in the electron-transfer mechanism . European Journal of Biochemistry . 207 . 1 . 61–8 . July 1992 . 1321049 . 10.1111/j.1432-1033.1992.tb17020.x . free .
7. Iobbi-Nivol C, Santini CL, Blasco F, Giordano G . Purification and further characterization of the second nitrate reductase of Escherichia coli K12 . European Journal of Biochemistry . 188 . 3 . 679–87 . March 1990 . 2139607 . 10.1111/j.1432-1033.1990.tb15450.x . free .