Nicotinamidase Explained

nicotinamidase
Ec Number:	3.5.1.19
Cas Number:	9033-32-3
Go Code:	0008936
Width:	270

In enzymology, a nicotinamidase is an enzyme that catalyzes the chemical reaction

nicotinamide + H₂O

\rightleftharpoons nicotinate + NH₃

Thus, the two substrates of this enzyme are nicotinamide and H₂O, whereas its two products are nicotinate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is nicotinamide amidohydrolase. Other names in common use include nicotinamide deaminase, nicotinamide amidase, and YNDase. This enzyme participates in nicotinate and nicotinamide metabolism.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

References

• Petrack B, Greengard P, Craston A, Sheppy F . 1965 . Nicotinamide deamidase from mammalian liver . J. Biol. Chem. . 240 . 1725 - 1730 . 14285515.
• Sarma DS, Rajalakshmi S, Sarma S . 1964 . Studies on the enzymes involved in nicotinamide adenine dinucleotide metabolism in Aspergillus niger . Biochim. Biophys. Acta . 81 . 2 . 311 - 322 . 10.1016/0926-6569(64)90047-1.