Mycothiol-dependent formaldehyde dehydrogenase explained

mycothiol-dependent formaldehyde dehydrogenase
Ec Number:	1.1.1.306
Go Code:	0050607

In enzymology, a mycothiol-dependent formaldehyde dehydrogenase is an enzyme that catalyzes the chemical reaction

formaldehyde + mycothiol + NAD⁺

\rightleftharpoons S-formylmycothiol + NADH + 2 H⁺

The 3 substrates of this enzyme are formaldehyde, mycothiol, and NAD⁺, whereas its 3 products are S-formylmycothiol, NADH, and H⁺.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase or S-(hydroxymethyl)mycothiol dehydrogenase.

Notes and References

1. Misset-Smits M, van Ophem PW, Sakuda S, Duine JA . Mycothiol, 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D- myo-inositol, is the factor of NAD/factor-dependent formaldehyde dehydrogenase . FEBS Letters . 409 . 2 . 221–2 . June 1997 . 9202149 . 10.1016/S0014-5793(97)00510-3 . free .
2. Norin A, Van Ophem PW, Piersma SR, Persson B, Duine JA, Jörnvall H . Mycothiol-dependent formaldehyde dehydrogenase, a prokaryotic medium-chain dehydrogenase/reductase, phylogenetically links different eukaroytic alcohol dehydrogenases--primary structure, conformational modelling and functional correlations . European Journal of Biochemistry . 248 . 2 . 282–9 . September 1997 . 9346279 . 10.1111/j.1432-1033.1997.00282.x . free .
3. Vogt RN, Steenkamp DJ, Zheng R, Blanchard JS . The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase . The Biochemical Journal . 374 . Pt 3 . 657–66 . September 2003 . 12809551 . 1223637 . 10.1042/BJ20030642 .
4. Rawat M, Av-Gay Y . Mycothiol-dependent proteins in actinomycetes . FEMS Microbiology Reviews . 31 . 3 . 278–92 . April 2007 . 17286835 . 10.1111/j.1574-6976.2006.00062.x . free .