Monosialoganglioside sialyltransferase explained

monosialoganglioside sialyltransferase
Ec Number:	2.4.99.2
Cas Number:	60202-12-2
Go Code:	0047288

In enzymology, a monosialoganglioside sialyltransferase is an enzyme that catalyzes the chemical reaction

CMP-N-acetylneuraminate + D-galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide

\rightleftharpoons CMP + N-acetylneuraminyl-D-galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide

The 2 substrates of this enzyme are CMP-N-acetylneuraminate and D-galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide, whereas its 2 products are CMP and N-acetylneuraminyl-D-galactosyl-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide.

This enzyme belongs to the family of transferases, specifically those glycosyltransferases that do not transfer hexosyl or pentosyl groups. The systematic name of this enzyme class is CMP-N-acetylneuraminate:D-galactosyl-N-acetyl-D-galactosaminyl-(N-ac etylneuraminyl)-D-galactosyl-D-glucosylceramide N-acetylneuraminyltransferase.

References

• J. Evan Sadler . Rearick JI, Sadler JE, Paulson JC, Hill RL . 1979 . Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland . J. Biol. Chem. . 254 . 4444–51 . 438198 . 11 .
• Yip MC . 1973 . The enzymic synthesis of disialoganglioside: rat brain cytidine-5'-monophospho-N-acetylneuraminic acid: monosialoganglioside (G M1) sialyltransferase . Biochim. Biophys. Acta . 306 . 298–306 . 4351506 . 2 . 10.1016/0005-2760(73)90235-X .