Methylenetetrahydrofolate—tRNA-(uracil-5-)-methyltransferase explained

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing)
Ec Number:	2.1.1.74
Cas Number:	74665-78-4
Go Code:	0047151

In enzymology, a methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH₂

\rightleftharpoons tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD

The 3 substrates of this enzyme are 5,10-methylenetetrahydrofolate, tRNA containing uridine at position 54, and FADH2, whereas its 3 products are tetrahydrofolate, tRNA containing ribothymidine at position 54, and FAD.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:tRNA (uracil-5-)-methyl-transferase. Other names in common use include (FADH2-oxidizing), folate-dependent ribothymidyl synthase, methylenetetrahydrofolate-transfer ribonucleate uracil, 5-methyltransferase, 5,10-methylenetetrahydrofolate:tRNA-UPsiC, and (uracil-5-)-methyl-transferase.

References

• Delk AS, Nagle DP, Rabinowitz JC . 1980 . Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2 . J. Biol. Chem. . 255 . 4387 - 90 . 6768721 . 10 .
• Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H . 1997 . Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs . J. Mol. Biol. . 274 . 505 - 18 . 9417931 . 10.1006/jmbi.1997.1417 . 4 .