Methanol dehydrogenase (nicotinoprotein) explained

Methanol dehydrogenase (nicotinoprotein)
Ec Number:	1.1.99.37

Methanol dehydrogenase (nicotinoprotein) (NDMA-dependent methanol dehydrogenase, nicotinoprotein methanol dehydrogenase, methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase) is an enzyme with systematic name methanol:acceptor oxidoreductase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

methanol + acceptor

\rightleftharpoons formaldehyde + reduced acceptor

This enzyme contains Zn²⁺ and Mg²⁺.

Notes and References

1. Vonck J, Arfman N, De Vries GE, Van Beeumen J, Van Bruggen EF, Dijkhuizen L . Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1 . The Journal of Biological Chemistry . 266 . 6 . 3949–54 . February 1991 . 1995642 .
2. Van Ophem PW, Van Beeumen J, Duine JA . Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica . European Journal of Biochemistry . 212 . 3 . 819–26 . March 1993 . 8385013 . 10.1111/j.1432-1033.1993.tb17723.x . free .
3. Bystrykh LV, Vonck J, van Bruggen EF, van Beeumen J, Samyn B, Govorukhina NI, Arfman N, Duine JA, Dijkhuizen L . Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N'-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19 . Journal of Bacteriology . 175 . 6 . 1814–22 . March 1993 . 8449887 . 203978 . 10.1128/jb.175.6.1814-1822.1993 .
4. Hektor HJ, Kloosterman H, Dijkhuizen L . Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus . The Journal of Biological Chemistry . 277 . 49 . 46966–73 . December 2002 . 12351635 . 10.1074/jbc.M207547200 . free .
5. Park H, Lee H, Ro YT, Kim YM . Identification and functional characterization of a gene for the methanol : N,N'-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803) . Microbiology . 156 . Pt 2 . 463–71 . February 2010 . 19875438 . 10.1099/mic.0.034124-0 . free .