Melilotate 3-monooxygenase explained

melilotate 3-monooxygenase
Ec Number:	1.14.13.4
Cas Number:	37256-72-7
Go Code:	0050091

In enzymology, a melilotate 3-monooxygenase is an enzyme that catalyzes the chemical reaction

3-(2-hydroxyphenyl)propanoate + NADH + H⁺ + O₂

\rightleftharpoons 3-(2,3-dihydroxyphenyl)propanoate + NAD⁺ + H₂O

The 4 substrates of this enzyme are 3-(2-hydroxyphenyl)propanoate, NADH, H⁺, and O₂, whereas its 3 products are 3-(2,3-dihydroxyphenyl)propanoate, NAD⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is 3-(2-hydroxyphenyl)propanoate,NADH:oxygen oxidoreductase (3-hydroxylating). Other names in common use include 2-hydroxyphenylpropionate hydroxylase, melilotate hydroxylase, 2-hydroxyphenylpropionic hydroxylase, and melilotic hydroxylase. This enzyme participates in phenylalanine metabolism. It employs one cofactor, FAD.

References

• Levy CC . 1967 . Melilotate hydroxylase. Purification of the enzyme and the nature of the prosthetic group . J. Biol. Chem. . 242 . 747 - 53 . 6017743 . 4 .
• Levy CC, Frost P . 1966 . The metabolism of coumarin by a microorganism. V. Melilotate hydroxylase . J. Biol. Chem. . 241 . 997 - 1003 . 4285850 . 4 .
• Strickland S, Massey V . 1973 . The purification and properties of the flavoprotein melilotate hydroxylase . J. Biol. Chem. . 248 . 2944 - 52 . 4348920 . 8 .
• Strickland S, Massey V . 1973 . The mechanism of action of the flavoprotein melilotate hydroxylase . J. Biol. Chem. . 248 . 2953 - 62 . 4348921 . 8 .