Mannosylglycoprotein endo-beta-mannosidase explained

Mannosylglycoprotein endo-beta-mannosidase
Ec Number:	3.2.1.152
Cas Number:	141176-95-6

Mannosylglycoprotein endo-beta-mannosidase (endo-beta-mannosidase) is an enzyme.^{[1] [2]} This enzyme catalyses the following chemical reaction:

Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences

The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein.

Notes and References

1. Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S . Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization . The Journal of Biological Chemistry . 279 . 37 . 38555–62 . September 2004 . 15247239 . 10.1074/jbc.m406886200 . free .
2. Sasaki A, Yamagishi M, Mega T, Norioka S, Natsuka S, Hase S . Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb . Journal of Biochemistry . 125 . 2 . 363–7 . February 1999 . 9990135 . 10.1093/oxfordjournals.jbchem.a022295 .