Mandelamide amidase explained

mandelamide amidase
Ec Number:	3.5.1.86
Go Code:	0050537

In enzymology, a mandelamide amidase is an enzyme that catalyzes the chemical reaction

(R)-mandelamide + H₂O

\rightleftharpoons (R)-mandelate + NH₃

Thus, the two substrates of this enzyme are (R)-mandelamide and H₂O, whereas its two products are (R)-mandelate and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is mandelamide hydrolase. This enzyme is also called Pseudomonas mandelamide hydrolase.

References

• Yamamoto K, Oishi K, Fujimatsu I, Komatsu K . 1991 . Production of R-(−)-mandelic acid from mandelonitrile by Alcaligenes faecalis ATCC 8750 . Appl. Environ. Microbiol. . 57 . 3028 - 32 . 1660699 . 10 . 183915 .