MRNA guanylyltransferase explained
| mRNA guanylyltransferase |
| Ec Number: | 2.7.7.50 |
| Cas Number: | 56941-23-2 |
| Go Code: | 0004484 |
In enzymology, a mRNA guanylyltransferase is an enzyme that catalyzes the chemical reaction
GTP + (5')ppPur-mRNA
diphosphate + G(5')pppPur-mRNA
Thus, the two substrates of this enzyme are GTP and (5')ppPur-mRNA, whereas its two products are diphosphate and G(5')pppPur-mRNA.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases).op The systematic name of this enzyme class is GTP:mRNA guanylyltransferase. Other names in common use include mRNA capping enzyme, messenger RNA guanylyltransferase, and Protein 2.
Structural studies
As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes,,,, and .
References
- Ensinger MJ, Martin SA, Paoletti E, Moss B . 1975 . Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus . Proc. Natl. Acad. Sci. U.S.A. . 72 . 2525 - 9 . 1058472 . 10.1073/pnas.72.7.2525 . 7 . 432801 . 1975PNAS...72.2525E . free .
- Groner Y, Gilboa E, Aviv H . 1978 . Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates . Biochemistry . 17 . 977 - 82 . 629955 . 10.1021/bi00599a005 . 6 .
- Itoh N, Yamada H, Kaziro Y, Mizumoto K . 1987 . Messenger RNA guanylyltransferase from Saccharomyces cerevisiae Large scale purification, subunit functions, and subcellular localization . J. Biol. Chem. . 262 . 1989 - 95 . 3029058 . 5 . 10.1016/S0021-9258(18)61609-6 . free .
- Martin SA, Moss B . 1975 . Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions . J. Biol. Chem. . 250 . 9330 - 5 . 1194287 . 24 . 10.1016/S0021-9258(19)40647-9 . free .
- Martin SA, Paoletti E, Moss B . 1975 . Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions . J. Biol. Chem. . 250 . 9322 - 9 . 1194286 . 24 . 10.1016/S0021-9258(19)40646-7 . free .
