Lysyl endopeptidase explained

Lysyl endopeptidase (R)
Ec Number:	3.4.21.50
Cas Number:	123175-82-6

Lysyl endopeptidase (Achromobacter proteinase I, Achromobacter lyticus alkaline proteinase I, protease I, achromopeptidase, lysyl bond specific proteinase, and caseinase) is an enzyme.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

Preferential cleavage: Lys-, including -Lys-Pro-

This enzyme is isolated from Lysobacter enzymogenes.

It is produced by the human body and by Achromobacter lyticus^[7] that can help break down the protein casein in milk into smaller peptides and amino acids.^[8]

Notes and References

1. Masaki T, Tanabe M, Nakamura K, Soejima M . Studies on a new proteolytic enzyme from A chromobacter lyticus M497-1. I. Purification and some enzymatic properties . Biochimica et Biophysica Acta . 660 . 1 . 44–50 . July 1981 . 6791693 . 10.1016/0005-2744(81)90106-6 .
2. Masaki T, Fujihashi T, Nakamura K, Soejima M . Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1. II. specificity and inhibition studies of Achromobacter protease I . Biochimica et Biophysica Acta . 660 . 1 . 51–5 . July 1981 . 6168293 . 10.1016/0005-2744(81)90107-8 .
3. Jekel PA, Weijer WJ, Beintema JJ . Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis . Analytical Biochemistry . 134 . 2 . 347–54 . October 1983 . 6359954 . 10.1016/0003-2697(83)90308-1 .
4. Elliott BW, Cohen C . Isolation and characterization of a lysine-specific protease from Pseudomonas aeruginosa . The Journal of Biological Chemistry . 261 . 24 . 11259–65 . August 1986 . 10.1016/S0021-9258(18)67377-6 . 3090046 . free .
5. Ohara T, Makino K, Shinagawa H, Nakata A, Norioka S, Sakiyama F . Cloning, nucleotide sequence, and expression of Achromobacter protease I gene . The Journal of Biological Chemistry . 264 . 34 . 20625–31 . December 1989 . 10.1016/S0021-9258(19)47109-3 . 2684982 . free .
6. Tsunasawa S, Masaki T, Hirose M, Soejima M, Sakiyama F . The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease . The Journal of Biological Chemistry . 264 . 7 . 3832–9 . March 1989 . 10.1016/S0021-9258(19)84926-8 . 2492988 . free .
7. Web site: BRENDA - Information on EC 3.4.21.50 - lysyl endopeptidase. www.brenda-enzymes.org. 2018-05-28.
8. Web site: Caseinase Definition and Examples - Biology Online Dictionary. 7 October 2019.