Lupeol synthase explained

Lupeol synthase
Ec Number:	5.4.99.41

Lupeol synthase (LUPI, BPW, RcLUS) is an enzyme with systematic name (3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, lupeol-forming).^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

(3S)-2,3-epoxy-2,3-dihydrosqualene

\rightleftharpoons lupeol

Also forms some beta-amyrin.

Notes and References

1. Herrera JB, Bartel B, Wilson WK, Matsuda SP . Cloning and characterization of the Arabidopsis thaliana lupeol synthase gene . Phytochemistry . 49 . 7 . 1905–11 . December 1998 . 9883589 . 10.1016/s0031-9422(98)00366-5 .
2. Shibuya M, Zhang H, Endo A, Shishikura K, Kushiro T, Ebizuka Y . Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases . European Journal of Biochemistry . 266 . 1 . 302–7 . November 1999 . 10542078 . 10.1046/j.1432-1327.1999.00875.x . free .
3. Segura MJ, Meyer MM, Matsuda SP . Arabidopsis thaliana LUP1 converts oxidosqualene to multiple triterpene alcohols and a triterpene diol . Organic Letters . 2 . 15 . 2257–9 . July 2000 . 10930257 . 10.1021/ol006016b .
4. Zhang H, Shibuya M, Yokota S, Ebizuka Y . Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla var. japonica: molecular evolution of oxidosqualene cyclases in higher plants . Biological & Pharmaceutical Bulletin . 26 . 5 . 642–50 . May 2003 . 12736505 . 10.1248/bpb.26.642 . free .
5. Hayashi H, Huang P, Takada S, Obinata M, Inoue K, Shibuya M, Ebizuka Y . Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra . Biological & Pharmaceutical Bulletin . 27 . 7 . 1086–92 . July 2004 . 15256745 . 10.1248/bpb.27.1086 . free .
6. Guhling O, Hobl B, Yeats T, Jetter R . Cloning and characterization of a lupeol synthase involved in the synthesis of epicuticular wax crystals on stem and hypocotyl surfaces of Ricinus communis . Archives of Biochemistry and Biophysics . 448 . 1–2 . 60–72 . April 2006 . 16445885 . 10.1016/j.abb.2005.12.013 .
7. Basyuni M, Oku H, Tsujimoto E, Kinjo K, Baba S, Takara K . Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae . The FEBS Journal . 274 . 19 . 5028–42 . October 2007 . 17803686 . 10.1111/j.1742-4658.2007.06025.x .