Lipoyl(octanoyl) transferase explained

Lipoyl(octanoyl) transferase
Ec Number:	2.3.1.181

In enzymology, a lipoyl(octanoyl) transferase is an enzyme that catalyzes the chemical reaction

octanoyl-[acyl-carrier-protein] + protein

\rightleftharpoons protein N⁶-(octanoyl)lysine + acyl carrier protein

Thus, the two substrates of this enzyme are octanoyl-[acyl-carrier-protein] and protein, whereas its two products are protein N6-(octanoyl)lysine and acyl carrier protein.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is octanoyl-[acyl-carrier-protein]:protein N-octanoyltransferase. Other names in common use include LipB, lipoyl (octanoyl)-[acyl-carrier-protein]-protein, N-lipoyltransferase, lipoyl (octanoyl)-acyl carrier protein:protein transferase, lipoate/octanoate transferase, lipoyltransferase, octanoyl-[acyl carrier protein]-protein N-octanoyltransferase, and lipoyl(octanoyl)transferase. This enzyme participates in lipoic acid metabolism.

References

• Broadwater JA, Haas JA, Fox BG, Booker SJ . 2005 . Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase . Protein Expr. Purif. . 39 . 269 - 82 . 15642479 . 2 . 10.1016/j.pep.2004.10.021 .
• Vanden Boom TJ, Reed KE, Cronan JE . 1991 . Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system . J. Bacteriol. . 173 . 6411 - 20 . 1655709 . 20 . 208974 .
• Jordan SW, Cronan JE . 1997 . A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria . J. Biol. Chem. . 272 . 17903 - 6 . 9218413 . 10.1074/jbc.272.29.17903 . 29 . free .
• Zhao X, Miller JR, Jiang Y, Marletta MA, Cronan JE . 2003 . Assembly of the covalent linkage between lipoic acid and its cognate enzymes . Chem. Biol. . 10 . 1293 - 302 . 14700636 . 10.1016/j.chembiol.2003.11.016 . 12 . free .
• Wada M, Yasuno R, Jordan SW, Cronan JE, Wada H . 2001 . Lipoic acid metabolism in Arabidopsis thaliana: cloning and characterization of a cDNA encoding lipoyltransferase . Plant Cell Physiol. . 42 . 650 - 6 . 11427685 . 10.1093/pcp/pce081 . 6 . free .
• Perham RN . 2000 . Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions . Annu. Rev. Biochem. . 69 . 961 - 1004 . 10966480 . 10.1146/annurev.biochem.69.1.961 .