Lipoate–protein ligase explained

Lipoate–protein ligase
Ec Number:	2.7.7.63
Cas Number:	144114-18-1

Lipoate–protein ligase (LplA, lipoate protein ligase, lipoate–protein ligase A, LPL, LPL-B) is an enzyme with systematic name ATP:lipoate adenylyltransferase.^{[1] [2] [3] [4] [5] [6] [7]} This enzyme catalyses the following chemical reaction

\rightleftharpoons diphosphate + lipoyl-AMP\rightleftharpoons protein N⁶-(lipoyl)lysine + AMP

This enzyme requires Mg²⁺ as a cofactor.

Notes and References

1. Morris TW, Reed KE, Cronan JE . Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. Molecular cloning and characterization of the lplA gene and gene product . The Journal of Biological Chemistry . 269 . 23 . 16091–100 . June 1994 . 8206909 .
2. Green DE, Morris TW, Green J, Cronan JE, Guest JR . Purification and properties of the lipoate protein ligase of Escherichia coli . The Biochemical Journal . 309 . 853–62 . August 1995 . 7639702 . 1135710 . 3 . 10.1042/bj3090853.
3. Zhao X, Miller JR, Jiang Y, Marletta MA, Cronan JE . Assembly of the covalent linkage between lipoic acid and its cognate enzymes . Chemistry & Biology . 10 . 12 . 1293–302 . December 2003 . 14700636 . 10.1016/j.chembiol.2003.11.016 . free .
4. Kim DJ, Kim KH, Lee HH, Lee SJ, Ha JY, Yoon HJ, Suh SW . Crystal structure of lipoate-protein ligase A bound with the activated intermediate: insights into interaction with lipoyl domains . The Journal of Biological Chemistry . 280 . 45 . 38081–9 . November 2005 . 16141198 . 10.1074/jbc.M507284200 . free .
5. Fujiwara K, Toma S, Okamura-Ikeda K, Motokawa Y, Nakagawa A, Taniguchi H . Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site . The Journal of Biological Chemistry . 280 . 39 . 33645–51 . September 2005 . 16043486 . 10.1074/jbc.M505010200 . free .
6. Jordan SW, Cronan JE . A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria . The Journal of Biological Chemistry . 272 . 29 . 17903–6 . July 1997 . 9218413 . 10.1074/jbc.272.29.17903 . free .
7. Perham RN . Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions . Annual Review of Biochemistry . 69 . 961–1004 . 2000 . 10966480 . 10.1146/annurev.biochem.69.1.961 .