Linoleate diol synthase explained

Linoleate diol synthase
Ec Number:	1.13.11.44

In enzymology, a linoleate diol synthase is an enzyme that catalyzes the chemical reaction

linoleate + O₂

\rightleftharpoons (9Z,12Z)-(7S,8S)-dihydroxyoctadeca-9,12-dienoate

Thus, the two substrates of this enzyme are linoleate and O₂, whereas its product is (9Z,12Z)-(7S,8S)-dihydroxyoctadeca-9,12-dienoate.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O₂. The systematic name of this enzyme class is linoleate:oxygen 7S,8S-oxidoreductase. This enzyme is also called linoleate (8R)-dioxygenase. This enzyme participates in linoleic acid metabolism.

References

• Brodowsky ID, Hamberg M, Oliw EH . 1992 . A linoleic acid (8R)-dioxygenase and hydroperoxide isomerase of the fungus Gaeumannomyces graminis. Biosynthesis of (8R)-hydroxylinoleic acid and (7S,8S)-dihydroxylinoleic acid from (8R)-hydroperoxylinoleic acid . J. Biol. Chem. . 267 . 14738 - 45 . 1634517 . 21 .
• Hamberg M, Zhang LY, Brodowsky ID, Oliw EH . 1994 . Sequential oxygenation of linoleic acid in the fungus Gaeumannomyces graminis: stereochemistry of dioxygenase and hydroperoxide isomerase reactions . Arch. Biochem. Biophys. . 309 . 77 - 80 . 8117115 . 10.1006/abbi.1994.1087 . 1 .
• Hörnsten . L . 1999 . Cloning of linoleate diol synthase reveals homology with prostaglandin H synthases . J. Biol. Chem. . 274 . 28219 - 24 . 10497176 . 10.1074/jbc.274.40.28219 . Su . C . Osbourn . AE . Garosi . P . Hellman . U . Wernstedt . C . Oliw . EH . 40 . free .
• Oliw EH, Su C, Skogstrom T, Benthin G . 1998 . Analysis of novel hydroperoxides and other metabolites of oleic, linoleic, and linolenic acids by liquid chromatography-mass spectrometry with ion trap MSn . Lipids . 33 . 843 - 52 . 9778131 . 10.1007/s11745-998-0280-0 . 9 .
• Su C, Oliw EH . 1996 . Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as a novel hemoprotein . J. Biol. Chem. . 271 . 14112 - 8 . 8662736 . 10.1074/jbc.271.24.14112 . 24 . free .
• Su C, Sahlin M, Oliw EH . 1998 . A protein radical and ferryl intermediates are generated by linoleate diol synthase, a ferric hemeprotein with dioxygenase and hydroperoxide isomerase activities . J. Biol. Chem. . 273 . 20744 - 51 . 9694817 . 10.1074/jbc.273.33.20744 . 33 . free .