Limonene-1,2-diol dehydrogenase explained

Limonene-1,2-diol dehydrogenase
Ec Number:	1.1.1.297

Limonene-1,2-diol dehydrogenase (NAD⁺-dependent limonene-1,2-diol dehydrogenase) is an enzyme with systematic name menth-8-ene-1,2-diol:NAD⁺ oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

menth-8-ene-1,2-diol + NAD⁺

\rightleftharpoons 1-hydroxymenth-8-en-2-one + NADH + H⁺ (general reaction)

(1) (1S,2S,4R)-menth-8-ene-1,2-diol + NAD⁺

\rightleftharpoons (1S,4R)-1-hydroxymenth-8-en-2-one + NADH + H⁺

(2) (1R,2R,4S)-menth-8-ene-1,2-diol + NAD⁺

\rightleftharpoons (1R,4S)-1-hydroxymenth-8-en-2-one + NADH + H⁺

While the enzyme from the Gram-positive bacterium Rhodococcus erythropolis DCL14 can use both (1S,2S,4R)- and (1R,2R,4S)-menth-8-ene-1,2-diol as substrate, activity is higher with (1S,2S,4R)-menth-8-ene-1,2-diol as substrate.

Notes and References

1. van der Werf MJ, Swarts HJ, de Bont JA . Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene . Applied and Environmental Microbiology . 65 . 5 . 2092–102 . May 1999 . 10224006 . 91303 .