Isotuberculosinol synthase explained

Isotuberculosinol synthase
Ec Number:	3.1.7.9

Isotuberculosinol synthase (Rv3378c) is an enzyme with systematic name tuberculosinyl diphosphate diphosphohydrolase (isotuberculosinol forming).^{[1] [2]} This enzyme catalyses the following chemical reaction

tuberculosinyl diphosphate + H₂O

\rightleftharpoons (13S)-isotuberculosinol + diphosphate

This enzyme is present in species of Mycobacterium that cause tuberculosis.

Notes and References

1. Nakano C, Ootsuka T, Takayama K, Mitsui T, Sato T, Hoshino T . Characterization of the Rv3378c gene product, a new diterpene synthase for producing tuberculosinol and (13R, S)-isotuberculosinol (nosyberkol), from the Mycobacterium tuberculosis H37Rv genome . Bioscience, Biotechnology, and Biochemistry . 75 . 1 . 75–81 . 2011 . 21228491 . 10.1271/bbb.100570 . free .
2. Hoshino T, Nakano C, Ootsuka T, Shinohara Y, Hara T . Substrate specificity of Rv3378c, an enzyme from Mycobacterium tuberculosis, and the inhibitory activity of the bicyclic diterpenoids against macrophage phagocytosis . Organic & Biomolecular Chemistry . 9 . 7 . 2156–65 . April 2011 . 21290071 . 10.1039/c0ob00884b .