Isopenicillin N N-acyltransferase explained
| isopenicillin N N-acyltransferase |
| Ec Number: | 2.3.1.164 |
| Cas Number: | 54576-90-8 |
| Go Code: | 0050640 |
In enzymology, an isopenicillin N N-acyltransferase is an enzyme that catalyzes the chemical reaction
phenylacetyl-CoA + isopenicillin N + H2O
CoA + penicillin G + L-2-aminohexanedioate
The 3 substrates of this enzyme are phenylacetyl-CoA, isopenicillin N, and H2O, whereas its 3 products are CoA, penicillin G, and L-2-aminohexanedioate.
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:isopenicillin N N-acyltransferase. Other names in common use include acyl-coenzyme A:isopenicillin N acyltransferase, and isopenicillin N:acyl-CoA: acyltransferase. This enzyme participates in hydrophobic penicillins biosynthesis.
References
- Tobin MB, Fleming MD, Skatrud PL, Miller JR . 1990 . Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli . J. Bacteriol. . 172 . 5908 - 14 . 2120195 . 10 . 526911 .
- Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ . Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry . Biochem. J. . 294 . 357 - 63 . 8396910 . 1134462 .
Further reading
- Aplin. Robin T.. Baldwin. Jack E.. Cole. Stephen C.J.. Sutherland. John D.. Tobin. Matthew B.. On the production of α,β-heterodimeric acyl-coenzyme A: isopenicillin N-acyltransferase of Penicillium chrysogenum. FEBS Letters. 319. 1-2. 1993. 166–170. 0014-5793. 10.1016/0014-5793(93)80060-8. free.
- Fernández FJ, Gutierrez S, Velasco J, Montenegro E, Marcos AT, Martín JF . Molecular characterization of three loss-of-function mutations in the isopenicillin N-acyltransferase gene (penDE) of Penicillium chrysogenum . . 176 . 16 . 4941–8 . August 1994 . 7519594 . 196331 .
- ALVAREZ. Emilio. MEESSCHAERT. Boudewijn. MONTENEGRO. Eduardo. GUTIERREZ. Santiago. DIEZ. Bruno. BARREDO. Jose L.. MARTIN. Juan F.. The isopenicillin-N acyltransferase of Penicillium chrysogenum has isopenicillin-N amidohydrolase, 6-aminopenicillanic acid acyltransferase and penicillin amidase activities, all of which are encoded by the single penDE gene. European Journal of Biochemistry. 215. 2. 1993. 323–332. 0014-2956. 10.1111/j.1432-1033.1993.tb18038.x. free.
