Isoleucine—tRNA ligase explained

Isoleucine—tRNA ligase
Ec Number:	6.1.1.5
Cas Number:	9030-96-0
Go Code:	0004822

In enzymology, an isoleucine—tRNA ligase is an enzyme that catalyzes the chemical reaction

ATP + L-isoleucine + tRNAIle

\rightleftharpoons AMP + diphosphate + L-isoleucyl-tRNAIle

The 3 substrates of this enzyme are ATP, L-isoleucine, and tRNA(Ile), whereas its 3 products are AMP, diphosphate, and L-isoleucyl-tRNA(Ile).

This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-isoleucine:tRNAIle ligase (AMP-forming). Other names in common use include isoleucyl-tRNA synthetase, isoleucyl-transfer ribonucleate synthetase, isoleucyl-transfer RNA synthetase, isoleucine-transfer RNA ligase, isoleucine-tRNA synthetase, and isoleucine translase. This enzyme participates in valine, leucine and isoleucine biosynthesis and aminoacyl-trna biosynthesis.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,,, and .

References

• ALLEN EH, GLASSMAN E, SCHWEET RS . 1960 . Incorporation of amino acids into ribonucleic acid. I. The role of activating enzymes . J. Biol. Chem. . 235 . 4 . 1061 - 7 . 10.1016/S0021-9258(18)69479-7 . free . 13792726 .
• Berg P, Bergmann FH, Ofengand EJ, Dieckmann M . 1961 . The enzymic synthesis of amino acyl derivatives of ribonucleic acid I. The mechanism of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid formation . J. Biol. Chem. . 236 . 1726 - 1734 . 10.1016/S0021-9258(19)63293-X . free .
• Bergmann FH, Berg P, Dieckmann M . 1961 . The enzymic synthesis of amino acyl derivatives of ribonucleic acid II. The preparation of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid synthetases from Escherichia coli . J. Biol. Chem. . 236 . 1735 - 1740 . 10.1016/S0021-9258(19)63294-1 . free .