Isoflavone 7-O-methyltransferase explained
| Isoflavone 7-O-methyltransferase |
| Ec Number: | 2.1.1.150 |
| Cas Number: | 136111-54-1 |
In enzymology, an isoflavone 7-O-methyltransferase is an enzyme that catalyzes the chemical reaction
S-adenosyl-L-methionine + a 7-hydroxyisoflavone
S-adenosyl-L-homocysteine + a 7-methoxyisoflavone
Thus, the two substrates of this enzyme are S-adenosyl methionine and 7-hydroxyisoflavone, whereas its two products are S-adenosylhomocysteine and 7-methoxyisoflavone.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase. This enzyme participates in isoflavonoid biosynthesis.
References
- Edwards R, Dixon RA. 1991 . Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa . . 30 . 2597 - 2606 . 10.1016/0031-9422(91)85107-B . 8 .
- He XZ, Dixon RA . 2000 . Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa . Plant Cell . 12 . 1689 - 702 . 11006341 . 10.1105/tpc.12.9.1689 . 9 . 149079 .
- He XZ, Dixon RA . 1996 . Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.) . Arch. Biochem. Biophys. . 336 . 121 - 9 . 8951042 . 10.1006/abbi.1996.0539 . 1 .
- He XZ, Reddy JT, Dixon RA . 1998 . Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase . Plant Mol. Biol. . 36 . 43 - 54 . 9484461 . 10.1023/A:1005938121453 . 1 . 22861966 .
- Liu CJ, Dixon RA . 2001 . Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis . Plant Cell . 13 . 2643 - 58 . 11752378 . 10.1105/tpc.13.12.2643 . 12 . 139479 .
- Zubieta C, He XZ, Dixon RA, Noel JP . 2001 . Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases . Nat. Struct. Biol. . 8 . 271 - 9 . 11224575 . 10.1038/85029 . 3 . 36170487 .
- Christensen AB, Gregersen PL, Olsen CE, Collinge DB . 1998 . A flavonoid 7-O-methyltransferase is expressed in barley leaves in response to pathogen attack . Plant Mol. Biol. . 36 . 219 - 27 . 9484434 . 10.1023/A:1005985609313 . 2 . 23711170 .
