Isoeugenol synthase explained

Isoeugenol synthase
Ec Number:	1.1.1.319

Isoeugenol synthase (IGS1, t-anol/isoeugenol synthase 1) is an enzyme with systematic name eugenol:NADP⁺ oxidoreductase (coniferyl acetate reducing).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction.^[4]

isoeugenol + acetate + NADP⁺

\rightleftharpoons coniferyl acetate + NADPH + H⁺

The enzyme acts in the reverse direction.

Dexter et al., 2007 finds that the substrate is coniferyl acetate.^[5]

Notes and References

1. Koeduka T, Fridman E, Gang DR, Vassão DG, Jackson BL, Kish CM, Orlova I, Spassova SM, Lewis NG, Noel JP, Baiga TJ, Dudareva N, Pichersky E . Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester . Proceedings of the National Academy of Sciences of the United States of America . 103 . 26 . 10128–33 . June 2006 . 16782809 . 1502517 . 10.1073/pnas.0603732103 . free .
2. Koeduka T, Louie GV, Orlova I, Kish CM, Ibdah M, Wilkerson CG, Bowman ME, Baiga TJ, Noel JP, Dudareva N, Pichersky E . The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages . The Plant Journal . 54 . 3 . 362–74 . May 2008 . 18208524 . 2741023 . 10.1111/j.1365-313X.2008.03412.x .
3. Koeduka T, Baiga TJ, Noel JP, Pichersky E . Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain . Plant Physiology . 149 . 1 . 384–94 . January 2009 . 18987218 . 2613694 . 10.1104/pp.108.128066 .
4. Gupta AK, Schauvinhold I, Pichersky E, Schiestl FP . Eugenol synthase genes in floral scent variation in Gymnadenia species . Functional & Integrative Genomics . 14 . 4 . 779–88 . December 2014 . 25239559 . 10.1007/s10142-014-0397-9 . 20.500.11850/91540 . 17207240 . free .

5. Vogt . Thomas . Phenylpropanoid Biosynthesis . . 3 . 1 . 2010 . 39503278 . 20035037 . 10.1093/mp/ssp106 . 2–20. free .

   This review cites this research.

   Dexter . Richard . Qualley . Anthony . Kish . Christine M. . Ma . Choong Je . Koeduka . Takao . Nagegowda . Dinesh A. . Dudareva . Natalia . Pichersky . Eran . Clark . David . Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol . . 49 . 2 . 2006-12-07 . 6714361 . 10.1111/j.1365-313x.2006.02954.x . 265–275. 17241449 . 2027.42/74478 . free .