Indoleacetate—lysine synthetase explained

Indoleacetate—lysine synthetase
Ec Number:	6.3.2.20
Cas Number:	103537-15-1
Go Code:	0047721

In enzymology, an indoleacetate—lysine synthetase is an enzyme that catalyzes the chemical reaction

ATP + (indol-3-yl)acetate + L-lysine

\rightleftharpoons ADP + phosphate + N₆-[(indol-3-yl)acetyl]-L-lysine

The 3 substrates of this enzyme are ATP, (indol-3-yl)acetate, and L-lysine, whereas its 3 products are ADP, phosphate, and N6-[(indol-3-yl)acetyl]-L-lysine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is (indol-3-yl)acetate:L-lysine ligase (ADP-forming). This enzyme is also called indoleacetate:L-lysine ligase (ADP-forming).

References

• Glass NL, Kosuge T . 1986 . Cloning of the gene for indoleacetic acid-lysine synthetase from Pseudomonas syringae subsp. savastanoi . J. Bacteriol. . 166 . 598 - 603 . 3084452 . 2 . 214646 .
• Hutzinger O, Kosuge T . 1968 . Microbial synthesis and degradation of indole-3-acetic acid. 3. The isolation and characterization of indole-3-acetyl-epsilon-L-lysine . Biochemistry . 7 . 601 - 5 . 5644130 . 10.1021/bi00842a013 . 2 .