Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) explained

Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)
Ec Number:	6.3.5.9
Cas Number:	132053-22-6
Go Code:	0043802

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) is an enzyme that catalyzes the chemical reaction

2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H₂O

\rightleftharpoons 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

The four substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H₂O; its four products are ADP, phosphate, hydrogenobyrinic acid a,c-diamide, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming). This enzyme is also called CobB and is part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in aerobic bacteria.

See also

• Cobalamin biosynthesis

References

• Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F . 1990 . Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans . J. Bacteriol. . 172 . 6239 - 44 . 2172209 . 11 . 526805 .
• Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC . 2002 . The biosynthesis of adenosylcobalamin (vitamin B12) . Nat. Prod. Rep. . 19 . 390 - 412 . 12195810 . 10.1039/b108967f . 4 .