Hydrogenase (NAD+, ferredoxin) explained

Hydrogenase (NAD^+, ferredoxin)
Ec Number:	1.12.1.4

Hydrogenase (NAD+, ferredoxin) (bifurcating [FeFe] hydrogenase) is an enzyme with systematic name hydrogen:NAD⁺, ferredoxin oxidoreductase.^{[1] [2]} This enzyme catalyses the following chemical reaction

2 H₂ + NAD⁺ + 2 oxidized ferredoxin

\rightleftharpoons 5 H⁺ + NADH + 2 reduced ferredoxin

The enzyme from Thermotoga maritima contains a [Fe-Fe] cluster (H-cluster) and iron-sulfur clusters. I

Notes and References

1. Verhagen MF, O'Rourke T, Adams MW . The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization . Biochimica et Biophysica Acta . 1412 . 3 . 212–29 . August 1999 . 10482784 . 10.1016/s0005-2728(99)00062-6 . free .
2. Schut GJ, Adams MW . The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production . Journal of Bacteriology . 191 . 13 . 4451–7 . July 2009 . 19411328 . 2698477 . 10.1128/JB.01582-08 .