Histone 3′ UTR stem-loop explained
| Histone 3′ UTR stem-loop |
| Symbol: | Histone3 |
| Rfam: | RF00032 |
| Rna Type: | Cis-reg |
| Tax Domain: | Eukaryota |
The histone 3′ UTR stem-loop is an RNA element involved in nucleocytoplasmic transport of the histone mRNAs, and in the regulation of stability and of translation efficiency in the cytoplasm. The mRNAs of metazoan histone genes lack polyadenylation and a poly-A tail, instead 3′ end processing occurs at a site between this highly conserved stem-loop and a purine rich region around 20 nucleotides downstream (the histone downstream element, or HDE). The stem-loop is bound by a 31 kDa stem-loop binding protein (SLBP - also termed the histone hairpin binding protein, or HBP). Together with U7 snRNA binding of the HDE, SLBP binding nucleates the formation of the processing complex.
References
- Williams AS, Marzluff WF . The sequence of the stem and flanking sequences at the 3′ end of histone mRNA are critical determinants for the binding of the stem-loop binding protein . Nucleic Acids Research . 23 . 4 . 654–662 . February 1995 . 7899087 . 306734 . 10.1093/nar/23.4.654 .
- Zanier K, Luyten I, Crombie C, Muller B, Schümperli D, Linge JP, Nilges M, Sattler M . Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression . RNA . 8 . 1 . 29–46 . January 2002 . 11871659 . 1370228 . 10.1017/s1355838202014061 .
- Zhang M, Lam TT, Tonelli M, Marzluff WF, Thapar R . Interaction of the histone mRNA hairpin with stem-loop binding protein (SLBP) and regulation of the SLBP-RNA complex by phosphorylation and proline isomerization . Biochemistry . 51 . 15 . 3215–3231 . April 2012 . 22439849 . 3328597 . 10.1021/bi2018255 .
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