Histochemical tracer explained

A histochemical tracer is a compound used to reveal the location of cells and track neuronal projections. A neuronal tracer may be retrograde, anterograde, or work in both directions. A retrograde tracer is taken up in the terminal of the neuron and transported to the cell body, whereas an anterograde tracer moves away from the cell body of the neuron.

List

• DiI (DiC18(3)) - retrograde and anterograde
• Diamidino yellow
• Fast blue
• Horseradish peroxidase - retrograde
• Cholera toxin B^{[1] [2] [3]} - retrograde and anterograde
• Pseudorabies virus
• Hydroxystilbamidine - retrograde
• Texas Red
• Fluorescein isothiocyanate

Notes and References

1. Kreier F, Kap YS, Mettenleiter TC, van Heijningen C, van der Vliet J, Kalsbeek A, Sauerwein HP, Fliers E, Romijn JA, Buijs RM . Tracing from fat tissue, liver, and pancreas: a neuroanatomical framework for the role of the brain in type 2 diabetes . Endocrinology . 147 . 3 . 1140–1147 . March 2006 . 16339209 . 10.1210/en.2005-0667 .
2. Angelucci A, Clascá F, Sur M . Anterograde axonal tracing with the subunit B of cholera toxin: a highly sensitive immunohistochemical protocol for revealing fine axonal morphology in adult and neonatal brains . Journal of Neuroscience Methods . 65 . 1 . 101–112 . March 1996 . 8815303 . 10.1016/0165-0270(95)00155-7 .
3. Coleman JE, Law K, Bear MF . Anatomical origins of ocular dominance in mouse primary visual cortex . Neuroscience . 161 . 2 . 561–571 . June 2009 . 19327388 . 2735235 . 10.1016/j.neuroscience.2009.03.045 .