Hippurate hydrolase explained

hippurate hydrolase
Ec Number:	3.5.1.32
Cas Number:	37278-43-6
Go Code:	0047980

In enzymology, a hippurate hydrolase is an enzyme that catalyzes the chemical reaction

hippurate + H₂O

\rightleftharpoons benzoate + glycine

Thus, the two substrates of this enzyme are hippurate and H₂O, whereas its two products are benzoate and glycine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-benzoylamino-acid amidohydrolase. This enzyme participates in phenylalanine metabolism.

Hippurate hydrolysis test is used in the presumptive identification of Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and group B streptococci by detecting the ability of the organism to hydrolyze hippurate.^[1]

References

• Tsoi E, Akmal S, Geerts L, Jeffery B, Nicolaides KH . 2006 . Sonographic measurement of cervical length and fetal fibronectin testing in threatened preterm labor . Ultrasound Obstet. Gynecol. . 27 . 368 - 72 . 16526097 . 10.1002/uog.2723 . 4 . free .
• Tsoi E, Akmal S, Geerts L, Jeffery B, Nicolaides KH . 2006 . Sonographic measurement of cervical length and fetal fibronectin testing in threatened preterm labor . Ultrasound Obstet. Gynecol. . 27 . 368 - 72 . 16526097 . 10.1002/uog.2723 . 4 . free .
• Rohrmann K, Niemann R, Buddecke E . 1985 . Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate . Eur. J. Biochem. . 148 . 463 - 9 . 3922754 . 10.1111/j.1432-1033.1985.tb08862.x . 3 . free .

Notes and References

1. https://hardydiagnostics.com/z18