High-mannose-oligosaccharide β-1,4-N-acetylglucosaminyltransferase explained

High-mannose-oligosaccharide β-1,4-N-acetylglucosaminyltransferase
Ec Number:	2.4.1.197
Cas Number:	123425-54-7

High-mannose-oligosaccharide β-1,4-N-acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-oligosaccharide acetylglucosaminyltransferase, acetylglucosamine-oligosaccharide acetylglucosaminyltransferase, UDP-GlcNAc:oligosaccharide beta-N-acetylglucosaminyltransferase, UDP-N-acetyl-D-glucosamine:high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:high-mannose-oligosaccharide 4-beta-N-acetylglucosaminyltransferase.^[1] This enzyme catalyses the following chemical reaction

Transfers an N-acetyl-D-glucosamine residue from UDP-N-acetyl-D-glucosamine to the 4-position of a mannose linked alpha-(1->6) to the core mannose of high-mannose oligosaccharides produced by Dictyostelium discoideum

The activity of the intersecting mannose residue as acceptor is dependent on two other mannose residues attached by alpha-1,3 and alpha-1,6 links.

Notes and References

1. Sharkey DJ, Kornfeld R . Identification of an N-acetylglucosaminyltransferase in Dictyostelium discoideum that transfers an "intersecting" N-acetylglucosamine residue to high mannose oligosaccharides . The Journal of Biological Chemistry . 264 . 18 . 10411–9 . June 1989 . 2525124 .