Haptoglobin-related protein explained

Haptoglobin-related protein (Hpr) is a serum protein that binds to haemoglobin of red blood cells and is present only in primates.[1] It acts as a molecule of innate immunity in association with apolipoprotein L1 (ApoL 1)-containing high-density lipoprotein (HDL) particles.[2] In humans, together with related serum protein, haptoglobin, it acts as a cell-killing agent as part of the trypanolytic factor against the protozoan parasite Trypanosoma brucei thereby providing natural resistance to African sleeping sickness.[3] It is produced from the gene HPR that is located on the long arm of chromosome 16 within the HP (for haptoglobin) gene cluster.[4]

History

Haptoglobin was discovered as a "plasma substance" in 1938 by French biochemists Max-Fernand Jayle and Michel Polonovski.[5] [6] The gene (later denoted as HP or Hp) was identified by British biochemist Oliver Smithies and his mentor, Canadian geneticist Norma Ford Walker in 1956.[7] [8] Smithies and Walker discovered that the gene could exist in two allelic autosomal genes, Hp1and Hp2.[9] Additional allele and associated genes were subsequently identified.

In 1983, Italian geneticist Riccardo Cortese and his team, led by Giovanni Raugei, sequenced the human Hp gene and discovered that there is a closely related gene in the vicinity.[10] As they reported: "Southern blot analysis provides evidence for the presence of more than one haptoglobin gene per haploid genome and confirms that there is restriction site polymorphism at this locus."[11] The next year, Smithies team, then at the University of Wisconsin, US, identified the same new gene and gave the name Hpr (for haptoglobin-related).[12] The protein, Hpr, was determined by New York University Medical Center scientists Madhavi Muranjan, Victor Nussenzweig and Stephen Tomlinson in 1998.

Structure

Hpr is 45-kDa in molecular size. It is structurally similar to haptoglobin with over 90% amino acid identity but in lesser concentration in the blood. Like haptoglobin, it is composed of α- and β-chain which are connected through a disulfide bond.[13] It lacks a glycosylation site and a cysteine involved in inter-α-chain bonding that are present in haptoglobin. Haptoglobin has either 1 (Hp1 genotype) or 2 (Hp2 genotype) of such cysteines. The α-chain of Hpr contains a hydrophobic signal peptide, which is absent in haptoglobin. The signal peptide makes Hpr associated with ApoL 1.

The HPR gene originated from duplication of the HP gene and is present at 2.2 kilobase pairs downstream of the HP gene on the long arm of chromosome 16 in humans. HPR is 94% similar in DNA sequence to HP gene. The gene is also present in apes and Old World monkeys in which it is created from a gene triplication (additional HP gene is present) during early evolution of the primate group. Some humans have additional copy of HPR gene. The gene product has 28-amino acid differences, 16 of which occur in the β chain. HPR has longer intron, 9.5 kilobase pairs compared to 1.3 kilobase pairs of that of haptoglobin. It contains a retrovirus-like element that is not found in haptoglobin.[14]

Function

Haptoglobin is known to be a high affinity-binding protein for haemoglobin during red blood cell destruction (haemolysis). Since Hpr is an accessory protein, it was initially believed that it that does not bind haemoglobin.[15] However, an experiment in 2006 showed that it binds to haemoglobin with same affinity as haptoglobin. Unlike haptoglobin which binds to the scavenger receptor CD163, a protein on macrophages that is critical for eliminating bacterial infection,[16] Hpr has no affinity for the receptor indicating that its primary role is different.[17]

The major function of Hpr is protection from infection with Trypanosoma brucei to provide natural resistance to African sleeping sickness. Together with haptolglobin and apoliproproteins, it makes up a trypanolytic factor TLF 1 in the blood of primates that can kill invading the animal strain of T. brucei (specifically T. b. brucei).[18] However, chimpanzees have mutated Hpr so that their serum cannot kill T. b. brucei.[19] The human strains, T. b. rhodesiense and T. b. gambiense have acquired resistance to TLF indicating an evolutionary arms race between primates and the protozoan parasite.[20] Hpr is also involved in TLF 2 in which its function is not yet understood.

External links

Notes and References

  1. McEvoy . S. M. . Maeda . N. . 1988-10-25 . Complex events in the evolution of the haptoglobin gene cluster in primates . The Journal of Biological Chemistry . 263 . 30 . 15740–15747 . 10.1016/S0021-9258(19)37650-1 . 0021-9258 . 3170608 . free.
  2. Nielsen . Marianne Jensby . Petersen . Steen Vang . Jacobsen . Christian . Oxvig . Claus . Rees . David . Møller . Holger Jon . Moestrup . Søren Kragh . 2006-10-15 . Haptoglobin-related protein is a high-affinity hemoglobin-binding plasma protein . Blood . 108 . 8 . 2846–2849 . 10.1182/blood-2006-05-022327 . 0006-4971 . 16778136 . free.
  3. Hardwick . Robert J. . Ménard . Anne . Sironi . Manuela . Milet . Jacqueline . Garcia . André . Sese . Claude . Yang . Fengtang . Fu . Beiyuan . Courtin . David . Hollox . Edward J. . 2014 . Haptoglobin (HP) and Haptoglobin-related protein (HPR) copy number variation, natural selection, and trypanosomiasis . Human Genetics . 133 . 1 . 69–83 . 10.1007/s00439-013-1352-x . 1432-1203 . 3898332 . 24005574.
  4. Koda . Y. . Soejima . M. . Yoshioka . N. . Kimura . H. . 1998 . The haptoglobin-gene deletion responsible for anhaptoglobinemia . American Journal of Human Genetics . 62 . 2 . 245–252 . 10.1086/301701 . 0002-9297 . 1376878 . 9463309.
  5. Shih . Andrew W. Y. . McFarlane . Andrew . Verhovsek . Madeleine . 2014 . Haptoglobin testing in hemolysis: measurement and interpretation . American Journal of Hematology . 89 . 4 . 443–447 . 10.1002/ajh.23623 . 1096-8652 . 24809098 . free.
  6. 1962-03-15 . Haptoglobins . New England Journal of Medicine . en . 266 . 11 . 569–570 . 10.1056/NEJM196203152661115 . 0028-4793.
  7. Smithies . O. . Walker . N. F. . 1956-09-29 . Notation for serum-protein groups and the genes controlling their inheritance . Nature . 178 . 4535 . 694–695 . 10.1038/178694a0 . 0028-0836 . 13369501. 1956Natur.178..694S .
  8. Mäkelä . O. . Eriksson . A.W. . Lehtovaara . Raimo . 2008-08-06 . On the inheritance of the haptoglobin serum groups . Acta Genetica et Statistica Medica . 9 . 2 . 149–166 . 10.1159/000151092 . 45103594 . 14420139. 0365-2785.
  9. Giblett . Eloise R. . 1964-01-01 . Variant Haptoglobin Phenotypes . Cold Spring Harbor Symposia on Quantitative Biology . en . 29 . 321–326 . 10.1101/SQB.1964.029.01.034 . 0091-7451 . 14278478.
  10. Bensi . G. . Raugei . G. . Klefenz . H. . Cortese . R. . 1985 . Structure and expression of the human haptoglobin locus. . The EMBO Journal . en . 4 . 1 . 119–126 . 10.1002/j.1460-2075.1985.tb02325.x . 554159 . 4018023.
  11. Raugei . G. . Bensi . G. . Colantuoni . V. . Romano . V. . Santoro . C. . Costanzo . F. . Cortese . R. . 1983-09-10 . Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA . Nucleic Acids Research . 11 . 17 . 5811–5819 . 10.1093/nar/11.17.5811 . 0305-1048 . 326319 . 6310515.
  12. Maeda . Nobuyo . Yang . Funmei . Barnett . Don R. . Bowman . Barbara H. . Smithies . Oliver . 1984 . Duplication within the haptoglobin Hp2 gene . Nature . en . 309 . 5964 . 131–135 . 10.1038/309131a0 . 1476-4687 . 6325933. 1984Natur.309..131M .
  13. Imrie . Heather J. . Fowkes . Freya J. I. . Migot-Nabias . Florence . Luty . Adrian J. F. . Deloron . Philippe . Hajduk . Stephen L. . Day . Karen P. . 2012 . Individual variation in levels of haptoglobin-related protein in children from Gabon . PLOS ONE . 7 . 11 . e49816 . 10.1371/journal.pone.0049816 . free . 1932-6203 . 3502254 . 23185445. 2012PLoSO...749816I .
  14. Maeda . N. . 1985-06-10 . Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element . The Journal of Biological Chemistry . 260 . 11 . 6698–6709 . 10.1016/S0021-9258(18)88836-6 . free . 0021-9258 . 2987228.
  15. Muranjan . M. . Nussenzweig . V. . Tomlinson . S. . 1998-02-13 . Characterization of the human serum trypanosome toxin, haptoglobin-related protein . The Journal of Biological Chemistry . 273 . 7 . 3884–3887 . 10.1074/jbc.273.7.3884 . 0021-9258 . 9461571 . free.
  16. Fabriek BO, van Bruggen R, Deng DM, Ligtenberg AJ, Nazmi K, Schornagel K, Vloet RP, Dijkstra CD, van den Berg TK . January 2009 . The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria . Blood . 113 . 4 . 887–92 . 10.1182/blood-2008-07-167064 . 18849484 . 4811741.
  17. Skytthe . Maria Kløjgaard . Sørensen . Anna Lahn . Hennig . Dorle . Sandberg . Maria Boysen . Rasmussen . Lars Melholt . Højrup . Peter . Møller . Holger J. . Skjødt . Karsten . Moestrup . Søren Kragh . Graversen . Jonas Heilskov . 2022-10-03 . Haptoglobin-related protein in human plasma correlates to haptoglobin concentrations and phenotypes . Scandinavian Journal of Clinical and Laboratory Investigation . en . 82 . 6 . 461–466 . 10.1080/00365513.2022.2122076 . 0036-5513 . 36129375. 252406321 .
  18. Drain . J. . Bishop . J. R. . Hajduk . S. L. . 2001-08-10 . Haptoglobin-related protein mediates trypanosome lytic factor binding to trypanosomes . The Journal of Biological Chemistry . 276 . 32 . 30254–30260 . 10.1074/jbc.M010198200 . 0021-9258 . 11352898 . free.
  19. Seed . J. R. . Sechelski . J. B. . Loomis . M. R. . 1990 . A survey for a trypanocidal factor in primate sera . The Journal of Protozoology . 37 . 5 . 393–400 . 10.1111/j.1550-7408.1990.tb01163.x . 0022-3921 . 2120433.
  20. Capewell . Paul . Cooper . Anneli . Clucas . Caroline . Weir . William . Macleod . Annette . 2015 . A co-evolutionary arms race: trypanosomes shaping the human genome, humans shaping the trypanosome genome . Parasitology . 142 . Suppl 1 . S108–119 . 10.1017/S0031182014000602 . 1469-8161 . 4413828 . 25656360.

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