Glycerol-3-phosphate dehydrogenase (NAD+) explained

glycerol-3-phosphate dehydrogenase (NAD^+)
Ec Number:	1.1.1.8
Cas Number:	9075-65-4
Go Code:	0004367
Glycerol-3-phosphate_dehydrogenase [NAD⁺]

In enzymology, a glycerol-3-phosphate dehydrogenase (NAD⁺) is an enzyme that catalyzes the chemical reaction

sn-glycerol 3-phosphate + NAD⁺

\rightleftharpoons glycerone phosphate + NADH + H⁺

The two substrates of this enzyme are sn-glycerol 3-phosphate and NAD⁺, whereas its 3 products are glycerone phosphate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is sn-glycerol-3-phosphate:NAD⁺ 2-oxidoreductase. Other names in common use include alpha-glycerol phosphate dehydrogenase (NAD⁺), alpha-glycerophosphate dehydrogenase (NAD⁺), glycerol 1-phosphate dehydrogenase, glycerol phosphate dehydrogenase (NAD⁺), glycerophosphate dehydrogenase (NAD⁺), hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, NAD⁺-alpha-glycerophosphate dehydrogenase, NAD⁺-dependent glycerol phosphate dehydrogenase, NAD⁺-dependent glycerol-3-phosphate dehydrogenase, NAD⁺-L-glycerol-3-phosphate dehydrogenase, NAD⁺-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase, and glycerol-3-phosphate dehydrogenase (NAD⁺). This enzyme participates in glycerophospholipid metabolism.

Structural studies

As of late 2007, 12 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,,,,, and .

References

• Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 7, Academic Press, New York, 1963, p. 85-96.
• Brosemer RW, Kuhn RW . 1969 . Comparative structural properties of honeybee and rabbit alpha-glycerophosphate dehydrogenases . Biochemistry . 8 . 2095 - 105 . 4307630 . 10.1021/bi00833a047 . 5 .
• O'Brien SJ, MacIntyre RJ . 1972 . The -glycerophosphate cycle in Drosophila melanogaster. I Biochemical and developmental aspects . Biochem. Genet. . 7 . 141 - 61 . 4340553 . 10.1007/BF00486085 . 2 . 22009695 .
• Warkentin DL, Fondy TP . 1973 . Isolation and characterization of cytoplasmic L-glycerol-3-phosphate dehydrogenase from rabbit-renal-adipose tissue and its comparison with the skeletal-muscle enzyme . Eur. J. Biochem. . 36 . 97 - 109 . 4200180 . 10.1111/j.1432-1033.1973.tb02889.x . 1 .
• Albertyn J, van Tonder A, Prior BA . 1992 . Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae . FEBS Lett. . 308 . 130 - 2 . 1499720 . 10.1016/0014-5793(92)81259-O . 2 . 39643279 .
• Koekemoer TC, Litthauer D, Oelofsen W . 1995 . Isolation and characterization of adipose tissue glycerol-3-phosphate dehydrogenase . Int. J. Biochem. Cell Biol. . 27 . 625 - 32 . 7671141 . 10.1016/1357-2725(95)00012-E . 6 .