Glutaryl-7-aminocephalosporanic-acid acylase explained
Glutaryl-7-aminocephalosporanic-acid acylase |
Ec Number: | 3.5.1.93 |
In enzymology, a glutaryl-7-aminocephalosporanic-acid acylase is an enzyme that catalyzes the chemical reaction
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
Thus, the two substrates of this enzyme are (7R)-7-(4-carboxybutanamido)cephalosporanate and H2O, whereas its two products are (7R)-7-aminocephalosporanate and glutarate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase. Other names in common use include 7beta-(4-carboxybutanamido)cephalosporanic acid acylase, cephalosporin C acylase, glutaryl-7-ACA acylase, CA, GCA, GA, cephalosporin acylase, glutaryl-7-aminocephalosporanic acid acylase, and GL-7-ACA acylase. This enzyme participates in penicillin and cephalosporin biosynthesis.
References
- Shimomura K . 1995 . High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176 . . 230 . 773–778 . 7607251 . 10.1111/j.1432-1033.1995.0773h.x . Saito . Y . Fujimura . T . Sasaki . H . Noguchi . Y . Yamada . H . Niwa . M . Shimomura . K . 2 . free .
- Kinoshita T, Tada T, Saito Y, Ishii Y, Sato A, Murata M . Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176 . . 56 . 458–459 . 10739919 . 10.1107/S0907444900000718 . Pt 4 .
- Monti D, Carrea G, Riva S, Baldaro E, Frare G . 2000 . Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase . . 70 . 239–244 . 10972935 . 10.1002/1097-0290(20001020)70:2<239::AID-BIT13>3.0.CO;2-I . 2 . free .
- Kwon TH, Rhee S, Lee YS, Park SS, Kim KH . 2000 . Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp GK16 . . 131 . 79–81 . 10945972 . 10.1006/jsbi.2000.4256 . 1 .
- Kim Y, Yoon K, Khang Y, Turley S, Hol WG . 2000 . The 2.0 A crystal structure of cephalosporin acylase . . 8 . 1059–1068 . 11080627 . 10.1016/S0969-2126(00)00505-0 . 10 . free .
- G . 2002 . Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp 130 . . 277 . 10256–10264 . 11782466 . 10.1074/jbc.M108683200 . Zeng . R . Ding . X . Mao . X . Ding . Y . Rao . Z . Xie . Y . Jiang . W . Zhao . G . 12 . free .
- Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH . 2003 . Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation . . 42 . 4084–4093 . 12680762 . 10.1021/bi027181x . 14 .