Glutamyl endopeptidase II explained

Glutamyl endopeptidase II
Ec Number:	3.4.21.82
Cas Number:	137010-42-5

Glutamyl endopeptidase II (GluSGP) is an enzyme.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

Preferential cleavage: -Glu- >> -Asp- . Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-Asp- and -Glu-Pro- bonds is slowThis enzyme is isolated from Streptomyces griseus.

Notes and References

1. Yoshida N, Tsuruyama S, Nagata K, Hirayama K, Noda K, Makisumi S . Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase) . Journal of Biochemistry . 104 . 3 . 451–6 . September 1988 . 3149277 .
2. Komiyama T, Bigler TL, Yoshida N, Noda K, Laskowski M . Replacement of P1 Leu18 by Glu18 in the reactive site of turkey ovomucoid third domain converts it into a strong inhibitor of Glu-specific Streptomyces griseus proteinase (GluSGP) . The Journal of Biological Chemistry . 266 . 17 . 10727–30 . June 1991 . 1674942 .
3. Nagata K, Yoshida N, Ogata F, Araki M, Noda K . Subsite mapping of an acidic amino acid-specific endopeptidase from Streptomyces griseus, GluSGP, and protease V8 . Journal of Biochemistry . 110 . 6 . 859–62 . December 1991 . 1794975 .
4. Svendsen I, Jensen MR, Breddam K . The primary structure of the glutamic acid-specific protease of Streptomyces griseus . FEBS Letters . 292 . 1-2 . 165–7 . November 1991 . 1959600 . 10.1016/0014-5793(91)80859-2 . free .
5. Breddam K, Meldal M . Substrate preferences of glutamic-acid-specific endopeptidases assessed by synthetic peptide substrates based on intramolecular fluorescence quenching . European Journal of Biochemistry . 206 . 1 . 103–7 . May 1992 . 1587264 . 10.1111/j.1432-1033.1992.tb16906.x . free .