Glutamate—tRNA(Gln) ligase explained

Glutamate—tRNA^Gln ligase
Ec Number:	6.1.1.24
Cas Number:	9068-76-2
Go Code:	0050561

In enzymology, a glutamate—tRNA^Gln ligase is an enzyme that catalyzes the chemical reaction

ATP + L-glutamate + tRNAGlx

\rightleftharpoons AMP + diphosphate + glutamyl-tRNAGlx

The 3 substrates of this enzyme are ATP, L-glutamate, and tRNAGlx, whereas its 3 products are AMP, diphosphate, and glutamyl-tRNAGlx.

This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-glutamate:tRNAGlx ligase (AMP-forming). This enzyme is also called glutamyl-tRNA synthetase. This enzyme participates in glutamate metabolism.

References

• Ibba M, Soll D . 2000 . Aminoacyl-tRNA synthesis . Annu. Rev. Biochem. . 69 . 617–50 . 10966471 . 10.1146/annurev.biochem.69.1.617 .
• Schmitt E, Moulinier L, Fujiwara S, Imanaka T, Thierry JC, Moras D . 1998 . Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation . EMBO J. . 17 . 5227–37 . 9724658 . 10.1093/emboj/17.17.5227 . 17 . 1170850 .
• Kim SI, Soll D . 1998 . Major identity element of glutamine tRNAs from Bacillus subtilis and Escherichia coli in the reaction with B. subtilis glutamyl-tRNA synthetase . Mol. Cells . 8 . 459–65 . 9749534 . 4 .