4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase explained

4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
Ec Number:	2.4.1.152
Cas Number:	111310-38-4
Go Code:	0017083

In enzymology, a 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase is an enzyme that catalyzes the chemical reaction

GDP-beta-L-fucose + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R

\rightleftharpoons

GDP + 1,4-beta-D-galactosyl-(alpha-1,3-L-fucosyl)-N-acetyl-D-glucosaminyl- R

Thus, the two substrates of this enzyme are GDP-beta-L-fucose and 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R. Its 3 products are GDP, 1,4-beta-D-galactosyl-(alpha-1,3-L-fucosyl)-N-acetyl-D-glucosaminyl-, and R.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-beta-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 3-alpha-L-fucosyltransferase. Other names in common use include:

Lewis-negative alpha-3-fucosyltransferase
plasma alpha-3-fucosyltransferase
guanosine diphosphofucose-glucoside alpha1→3-fucosyltransferase
galactoside 3-fucosyltransferase
GDP-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R
3-L-fucosyltransferase
GDP-beta-L-fucose:1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R, and
3-L-fucosyltransferase

This enzyme participates in 3 metabolic pathways: glycosphingolipid biosynthesis - neo-lactoseries, glycosphingolipid biosynthesis - globoseries, and glycan structures - biosynthesis 2.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

References

Johnson PH, Yates AD, Watkins WM . 1981 . Human salivary fucosyltransferases : evidence for two distinct alpha-3-L-fucosyltransferase activities one or which is associated with the Lewis blood group Le gene . Biochem. Biophys. Res. Commun. . 100 . 1611–8 . 7295318 . 10.1016/0006-291X(81)90703-8 . 4 .
Book: Schachter H, Narasimhan S, Gleeson P, Vella G . Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type . 1983 . Biomembranes Part L: Membrane Biogenesis: Processing and Recycling . 98 . 98–134 . 6366476 . 10.1016/0076-6879(83)98143-0 . Methods in Enzymology . 978-0-12-181998-9 .
Ma B, Wang G, Palcic MM, Hazes B, Taylor DE . 2003 . C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer . J. Biol. Chem. . 278 . 21893–900 . 12676935 . 10.1074/jbc.M301704200 . 24 . free .