Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase explained

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Ec Number:	2.4.1.288

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.^[1] ^[2] ^[3] This enzyme catalyses the following chemical reaction

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

\rightleftharpoons

28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

This enzyme is isolated from Mycobacterium tuberculosis.

Notes and References

Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL . Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase . Carbohydrate Research . 343 . 12 . 2130–9 . August 2008 . 18423586 . 10.1016/j.carres.2008.03.023 .
May JF, Splain RA, Brotschi C, Kiessling LL . A tethering mechanism for length control in a processive carbohydrate polymerization . Proceedings of the National Academy of Sciences of the United States of America . 106 . 29 . 11851–6 . July 2009 . 19571009 . 2715488 . 10.1073/pnas.0901407106 . 2009PNAS..10611851M . free .
Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK . Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan . The Journal of Biological Chemistry . 287 . 33 . 28132–43 . August 2012 . 22707726 . 3431634 . 10.1074/jbc.M112.347484 . free .