Galactan 5-O-arabinofuranosyltransferase explained

Galactan 5-O-arabinofuranosyltransferase
Ec Number:2.4.2.46

Galactan 5-O-arabinofuranosyltransferase (AftA, Rv3792) is an enzyme with systematic name galactofuranan:trans,octacis-decaprenylphospho-beta-D-arabinofuranose 5-O-alpha-D-arabinofuranosyltransferase.[1] This enzyme catalyses the following chemical reaction

Adds an alpha-D-arabinofuranosyl group from trans,octacis-decaprenylphospho-β-D-arabinofuranose at the 5-O-position of the eighth, tenth and twelfth galactofuranose unit of the galactofuranan chain of [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

This enzyme is isolated from Mycobacterium tuberculosis and Corynebacterium glutamicum.

Notes and References

  1. Alderwick LJ, Seidel M, Sahm H, Besra GS, Eggeling L . Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis . The Journal of Biological Chemistry . 281 . 23 . 15653–61 . June 2006 . 16595677 . 10.1074/jbc.M600045200 . free .