Fluoroacetyl-CoA thioesterase explained

Fluoroacetyl-CoA thioesterase
Ec Number:	3.1.2.29

The enzyme fluoroacetyl-CoA thioesterase (EC 3.1.2.29; systematic name fluoroacetyl-CoA hydrolase) catalyses the following reaction:^{[1] [2] [3]}

fluoroacetyl-CoA + H₂O

\rightleftharpoons fluoroacetate + CoA

Fluoroacetate is extremely toxic.

Notes and References

1. Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum . Meyer JJ, Grobbelaar N, Vleggaar R, Louw AI . J. Plant Physiol. . 1992 . 139 . 3 . 369–372 . 10.1016/s0176-1617(11)80352-4.
2. Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB . The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A . Chemistry & Biology . 13 . 5 . 475–84 . May 2006 . 16720268 . 10.1016/j.chembiol.2006.02.014 .
3. Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL . Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK . The Journal of Biological Chemistry . 285 . 29 . 22495–504 . July 2010 . 20430898 . 2903362 . 10.1074/jbc.m110.107177 . free .