Flavonoid 3'-monooxygenase explained

flavonoid 3'-monooxygenase
Ec Number:	1.14.14.82
Cas Number:	85340-98-3
Go Code:	0016711

In enzymology, a flavonoid 3'-monooxygenase (was wrongly classified as in the past) is an enzyme that catalyzes the chemical reaction^[1]

a flavonoid + NADPH + H⁺ + O₂

\rightleftharpoons a 3'-hydroxyflavonoid + NADP⁺ + H₂O

The 4 substrates of this enzyme are flavonoid, NADPH, H⁺, and O₂, whereas its 3 products are 3'-hydroxyflavonoid, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O₂ as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O₂ with NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is flavonoid,NADPH:oxygen oxidoreductase (3'-hydroxylating). Other names in common use include flavonoid 3'-hydroxylase, flavonoid 3-hydroxylase (erroneous), NADPH:flavonoid-3'-hydroxylase, and flavonoid 3-monooxygenase (erroneous). This enzyme participates in flavonoid biosynthesis.

Notes and References

1. Forkmann G, Heller W, Grisebach H . Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases . Z. Naturforsch. C: Biosci. . 35 . 1980 . 691–695.